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Rabbit Anti-Nrf2 antibody (bs-1074R)
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说明书: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1074R
英文名称 Nrf2
中文名称 核因子2相关因子2抗体
别    名 Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.  
Specific References  (25)     |     bs-1074R has been referenced in 25 publications.
[IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine  WB ;  Human.  
[IF=5.27] Lee, Bao-Hong, et al. "Ankaflavin: A natural novel PPAR?? agonist up-regulated Nrf2 to attenuate methylglyoxal (MG)-induced diabetes< i> in vivo."Free Radical Biology and Medicine (2012).y  WB ;  Rat.  
[IF=3.01] Bao-Hong, L. et al. "Graptopetalum paraguayense and resveratrol ameliorates 3 carboxymethyllysine (CML)-induced pancreas dysfunction and 4 hyperglycemia." Food and Chemical Toxicology. (2013).  WB ;  Mouse.  
[IF=3.01] Lee, Bao-Hong, et al. "Suppression of dimerumic acid on hepatic fibrosis caused from carboxymethyl-lysine (CML) by attenuating oxidative stress depends on Nrf2 activation in hepatic stellate cells (HSCs)." Food and Chemical Toxicology (201  WB ;  Mouse.  
[IF=3.01] Lee, Bao-Hong, et al. "< i> Graptopetalum paraguayense and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." Food and Chemical Toxicology 62 (2013): 492-498.  WB ;  Mouse.  
[IF=3.01] Lee, Bao-Hong, et al. "Suppression of dimerumic acid on hepatic fibrosis caused from carboxymethyl-lysine (CML) by attenuating oxidative stress depends on Nrf2 activation in hepatic stellate cells (HSCs)." Food and Chemical Toxicology 62 (2013): 413-419.  WB ;  Mouse.  
[IF=2.91] Hsu, Wei-Hsuan, et al. "Monascin and AITC attenuate methylglyoxal-induced PPARγ phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation." Journal of agricultural and food chemistry (2013).  WB ;  Rat.  
[IF=2.91] Lee, Bao-Hong, et al. "Effects of monascin on anti-inflammation mediated by Nrf2 activation in advanced glycation endproducts-treated THP-1 monocytes and methylglyoxal-treated Wistar rats." Journal of Agricultural and Food Chemistry (2013).  
[IF=2.74] Cheng, An-Sheng, Yu-Hsiang Cheng, and Tsu-Liang Chang. "Resveratrol protects RINm5F pancreatic cells from methylglyoxal-induced apoptosis." Journal of Functional Foods (2013).  WB ;  Rat.  
[IF=2.00] Wang, Yan, et al. "The Chronic Effects of Low Lead Level on The Expressions of Nrf2 and Mrp1 of The Testes in The Rats." Environmental Toxicology and Pharmacology (2012).  IHC-P ;  Rat.  
[IF=1.21] Lee, Chia-Chen, Bao-Hong Lee, and She-Ching Wu. "Actinidia callosa peel (kiwi fruit) ethanol extracts protected neural cells apoptosis induced by methylglyoxal through Nrf2 activation." Pharmaceutical Biology 0 (2014): 1-9.  other ;  
[IF=3.38] Cui, Y., et al. "Role of Nuclear Factor Erythroid 2-Like 2 in the Induction of Cytochrome P450 2a5 in Vivo of Nonalcoholic Fatty Liver Disease." Journal of Diabetes & Metabolism 6.488 (2015): 2.  WB ;  Mouse.  
[IF=3.83] Wang, Peng, et al. "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzymes expression via PI3K/AKT and ERK1/2 pathway." Biochimica et Biophysica Acta (BBA)-General Subjects (2015).  WB ;  Human.  
[IF=1.72] Yu, Haijie, et al. "Triptolide Attenuates Myocardial Ischemia/Reperfusion Injuries in Rats by Inducing the Activation of Nrf2/HO-1 Defense Pathway."Cardiovascular Toxicology (2015): 1-11.  WB ;  Rat.  
[IF=2.70] Lv, Runxiao, et al. "Neuroprotective effect of allicin in a rat model of acute spinal cord injury." Life Sciences (2015).  WB ;  Rat.  
[IF=2.62] Jiang, Wu, et al. "Protective Effects of Asiatic Acid Against Spinal Cord Injury-Induced Acute Lung Injury in Rats." Inflammation: (2016) 1-9.  WB ;  Rat.  
[IF=1.07] Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597.  WB ;  Human.  
[IF=0.87] Zeng, X-P., et al. "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." Transplantation Proceedings. Vol. 49. , 2017.  WB ;  Rat.  
[IF=2.19] Zhang, Jin-ming, et al. "Nrf2 Is Crucial For The Down-Regulation Of Cyp7a1 Induced By Arachidonic Acid In Hepg2 Cells." Environmental Toxicology and Pharmacology (2017).  WB ;  Human.  
[IF=2.55] Yu, Dan, et al. "In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats." International Immunopharmacology 47 (2017): 78-87.  IHC-P ;  Rat.  
[IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.  WB ;  Mouse.  
[IF=3.97] Zhao, Lei, et al. "Protective effects of Lactobacillus plantarum C88 on chronic ethanol-induced liver injury in mice." Journal of Functional Foods 35 (2017): 97-104.  WB ;  Rat.  
[IF=0.23] Tao, Kai, Jinghai Chen, and Lingyun Wang. "Effects of berberine on the expressions of NRF2 and HO-1 in endothelial cells of diabetic rat." Biomedical Research 28.9 (2017): 3860-3864.  WB, IHC-P ;  Rat.  
[IF=1.56] Zhang, Bing, et al. "Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells." Molecular Medicine Reports.  WB ;  Human.  
[IF=4.08] Cao, Zhen, et al. "Cyanidin Suppresses Autophagic Activity Regulating Chondrocyte Hypertrophic Differentiation." Journal of Cellular Physiology(2017).  WB ;  Mouse.  
研究领域 肿瘤  免疫学  染色质和核信号  合成与降解  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, 
产品应用 WB=1:100-1000 ELISA=1:1000-5000 IHC-P=1:100-1000 IHC-F=1:100-1000 IF=1:100-1000 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 68kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Nrf2:401-500/605 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Function:
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Subunit:
Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.

Tissue Specificity:
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Post-translational modifications:
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

Similarity:
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.

SWISS:
Q16236

Gene ID:
4780

Database links:

Entrez Gene: 4780 Human

Omim: 600492 Human

SwissProt: Q16236 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转录调节因子(Transcriptin Regulators) 核细胞系因子2相关因子Nrf2,是一种对氧化还原反应敏感的转录因子,可以通过正向促进基因的表达,来转录翻译产生一些抗氧化物,行使着异化解毒的酶类和药物的高效外排泵作用。
有学者认为:核因子2相关因子2蛋白在保护细胞免受环境毒性物质影响方面发挥着极其重要的作用,它指导某些基因促进细胞的防御工事,同时也刺激某些重要解毒酶对毒性物质,如黄曲霉素的解毒和排毒作用。
产品图片
Sample:
HepG2(Human) Cell Lysate at 40 ug
MCF-7(Human) Cell Lysate at 40 ug
Primary: Anti-Nrf2(bs-1074R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD
Sample:A549 (Human)Cell Lysate at 40 ug
Primary: Anti-Nrf2(bs-1074R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD
Sample:
Brain(Rat) lysate at 30ug;
Brain(Mouse) lysate at 30ug;
Primary: Anti-Nrf2 (bs-1074R) at 1:200 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution
Predicted band size : 66kD
Observed band size : 66kD
Primary: Anti-Nrf2 (bs-1074R) at 1:200
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000
Predicted band size : 66kD
Observed band size : 66kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nrf2 Polyclonal Antibody, Unconjugated(bs-1074R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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